- Uncle Tex as Mufasa
- Wilma as Sarabi
- The Great Gazoo as Rafiki
- Fred Flintstone as Zazu (both are vain and get grumpy)
- Barney Rubble as Adult Simba
- Betty as Adult Nala
- Xavier the Villian as Scar
- Vanellope Von Schweetz (from Wreck It Ralph) as Shenzi
- Shnookums (from The Shnookums and Meat Funny Cartoon Show) as Banzai
- Meat (from The Shnookums and Meat Funny Cartoon Show) as Ed
- Bam Bam as Young Simba
- Pebbles as Young Nala
- Pac-Man, Cylindria and Spiral (from Pac-Man and the Ghostly Adventures) as Timon and Pumbaa
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