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- Twist as Kuzco (Human)
- Underdog (from Underdog) as Kuzco (Llama)
- Shout as Pacha
- Catwoman (from Batman) as Yzma
- Harper as Kronk
- Kiki as Chicha
- Junior Beats as Chaca and Tipo
- Simon (from Alvin and The Chipmunks) as Bucky the Squirrel
- Ne-yo as Theme Song Guy
- Melody as Waitress
- Reed as Old Man
- Lucifer (from Cinderella) as Yzma (Cat)