- Basil of Baker Street - Darkwing Duck (Darkwing Duck)
- Dr. David Q. Dawson - Lauchpad McQuack (Ducktales/Darkwing Duck)
- Mouse with a crutch - ???
- Hiram Flaversham - Fenton Crackshell (Ducktales)
- Olivia Flaversham - Gosalyn Mallard (Darkwing Duck)
- Ratigan - Negaduck (Darkwing Duck)
- Fidget - Megavolt (Darkwing Duck)
- Toby - Spike (Tom and Jerry)
- Mrs. Judson - Mrs. Bertina Beakley (Ducktales)
- Ratigan's gang - Bushroot, Liquidator and Quackerjack (Darkwing Duck)
- Bartholomew - Sheldon J. Plankton (Spongebob Squarepants)
- Felicia - Jaws (Jaws)
- Barmaid - ???
- client from hampstead - ???
- Juggling Octopus - Fozzie Bear (The Muppet Movie)
- Frog and Salamander on Bike - ???
- sailors and bums -
- Miss Kitty - Morganna Macawber (Darkwing Duck)
- Miss Kitty's Sisters - ???
- Queen Moustoria - Minnie Mouse (Mickey, Goofy and Donald: The Three Musketeers)
- piano mouse - Donald Duck (Who Framed Roger Rabbit?)
- bully mouse - ???
- disguised criminal - Constantine the Frog (Muppets Most Wanted)
- queen's audience - The Muppets, Sesame Street gang
- Sherlock Holmes - Tigger (Winnie the Pooh)
- Dr. Watson - Winnie the Pooh (Winnie the Pooh)
- The Great Masked Mallard Detective Part 1 - Kidnapped/Main Titles
- The Great Masked Mallard Detective Part 2 - Launchpad (Dawson) Finds Gosalyn (Olivia)
- The Great Masked Mallard Detective Part 3 - Enter Darkwing Duck (Basil)
- The Great Masked Mallard Detective Part 4 - Enter Negaduck (Ratigan)
- The Great Masked Mallard Detective Part 5 - "The World's Greatest Criminal Mind"
- The Great Masked Mallard Detective Part 6 - The Unusual Footprints/Here's Spike (Toby)
- The Great Masked Mallard Detective Part 7 - At the Toy Store
- The Great Masked Mallard Detective Part 8 - Megavolt Kidnapped Gosalyn/The Chase
- The Great Masked Mallard Detective Part 9 - "There's Always a Chance, Doctor"/Reunion
- The Great Masked Mallard Detective Part 10 - Negaduck's Plan
- The Great Masked Mallard Detective Part 11 - Darkwing's Observation
- The Great Masked Mallard Detective Part 12 - At the Pub
- The Great Masked Mallard Detective Part 13 - "Let Me Be Good to You"
- The Great Masked Mallard Detective Part 14 - The Bar Fight/Following Megavolt
- The Great Masked Mallard Detective Part 15 - Darkwing Duck and Negaduck's Confrontation
- The Great Masked Mallard Detective Part 16 - Negaduck's Death Trap/"Goodbye So Soon"/The Queen's Doom
- The Great Masked Mallard Detective Part 17 -"We'll Set the Trap Off Now!"
- The Great Masked Mallard Detective Part 18 - At Buckingham Palace
- The Great Masked Mallard Detective Part 19 - The Big Ben Chase (Part 1)
- The Great Masked Mallard Detective Part 20 - The Big Ben Chase (Part 2)
- The Great Masked Mallard Detective Part 21 - "I'll Never Forget You"/A New Case
- The Great Masked Mallard Detective Part 22 - End Credits/"End Titles and Goodbye So Soon Reprise"
Megavolt/Fidget: (he laughs) Ah-ha! (he caught Gosalyn/Olivia as she screamed and has gotten a trap)
Gosalyn/Olivia: AAH! Hey, Let go of me, let me go!
Megavolt/Fidget: Gotcha!!! (Laughing)
Gosalyn/Olivia: Let me go!
Launchpad/Dawson: DW, over here!
(Darkwing Duck/Basil was so glad to see Launchpad/Dawson, Gosalyn/Olivia and Fenton Crackshell/Hiram. But then, Negaduck/Ratigan appears)
Negaduck/Ratigan: Grrrr!!! (Darkwing/Basil tries to escape) There's no escape this time, Darkwing Duck!
Negaduck/Ratigan: I won! Darkwing Duck is gone, gone, gone! (He evilly laughs)
Darkwing/Basil: On Contraire, mon fraire! (Negaduck/Ratigan stop laughing and look down there was Darkwing/Basil was still very much alive and hanging on the propeller part of the wrecked blimp) The game is not over yet!
(He ring the bell and Negaduck/Ratigan looked at the clock, the Big Ben's bell rings! Because the bell ring was so loud, Negaduck/Ratigan falls off Big Ben's hand)
Negaduck/Ratigan: AAAAAAHHH! (He grabs Darkwing/Basil. Negaduck/Ratigan was so heavy causing the blimp's ropes to the propeller breaks and then, the propeller part breaks) AAAAAAAAAAAAAAAAAAAAAAAAAAAHHHHHHH!!!!!!!
(Both Darkwing/Basil and Negaduck/Ratigan fell down into the mists below.)
Gosalyn/Olivia: NO! He couldn't be...
(Then, Gosalyn/Olivia only turned to her father, starting to cry.)
Gosalyn/Olivia: Oh, I can't believe he's...gone!
(Suddenly, there was a squeaking sound. It was Darkwing/Basil! He was still alive and riding on the blimp's propeller. Launchpad/Dawson, Fenton Crackshell/Hiram and Gosalyn/Olivia cheers.)
Plankton/Bartholomew: (Clearly drunked) To Negaduck, the world's Public enemy number two! (hiccups)
Negaduck/Ratigan: (spits out drink in shock and the Beagle Boys/Thugs/Quackerjack/Liquidator/Bushroot all gasp) What was THAT!? (Plankton/Bartholomew hiccups again) What did you call me?
Bushroot: Oh, he didn't mean it, Negaduck!
Quackerjack/Thug #3: It was just a slip of the tongue!
Negaduck/Ratigan: (angrily) I am NOT public enemy number two!
Drake the Penguin/Thug #2: 'Course you're not! You are a public enemy number one!!!
Dennis the Assassin/Thug #1: Yeah, that's right; right, Public enemy number one!!!
Ma Beagle/Thug #3: Yeah, number one!!!
Beagle Boy/Thug 4: Yes!!
Liquidator/Thug 5: Number one!!!
Negaduck/Ratigan: SHUT UP! (tosses Plankton/Bartholomew outside) Oh, my dear Plankton, I'm afraid that you've gone and upset me. (pulls a bell out of pocket) You know what happens when someone upsets me... (rings the bell)
Thugs/Beagle Boys/Quackerjack/Bushroot/Liquidator: (Cries in shock as a death bell rings)
Jaws/Felicia: (Steps out of a room; walk towards Plankton/Bartholomew)
Plankton/Bartholomew: (Unaware he's about to die; begins singing) Oh, Negaduck, Oh, Negaduck. You're the tops and that's that. (Hiccups) Oh, dear. (Resumes singing) To Negaduck, To Negaduck...
Beagle Boys/Thugs/Quackerjack/Liquidator/Bushroot: (Cries in fear)
Plankton/Bartholomew: (Has been snatched by the teeth of Jaws/Felicia) To Negaduck, The world's greatest... (gets eaten)
Thugs/Beagle Boys/Bushroot/Liquidator/Quackerjack: (Mourn fall of their friend)
Negaduck/Ratigan: (Lovingly) Oh, Jaws, my precious, my baby. Did Daddy's little honey-bun enjoy his tasty treat?
Negaduck/Ratigan: (Evil smile turns into frown; walks back inside) I trust there shall be no further interruptions. And now, as you were singing...?
Thugs/Beagle Boys/Liquidator/Bushroot/Quackerjack: (Hesitate to singing until Negaduck/Ratigan pulls his bell back out)
Negaduck/Ratigan: You knobs all knows the plan
Quackerjack/Beagle Boys/thugs/Bushroot/Liquidator: (saluted) Right, boss!