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- This is the Great Mouse Detective parody of film from 1986 by The Great Prehistoric Detective.
- Basil - Littlefoot (The Land Before Time)
- Dr Dawson - Tiger (An American Tail)
- Olivia - Buster (We're Back! A Dinosaur's Story)
- Hiram - Rex (We're Back! A Dinosaur's Story)
- Toby - Dodger (Oliver & Company)
- Fidget - Pterano (The Land Before Time)
- Ratigan - Professor Screweyes (We're Back! A Dinosaur's Story)
- Ms Judson - Ali (The Land Before Time)
- Ratigan's henchman - Louie, Cecilia, Tanya, Ducky, Petrie, Spike, and Cera (We're Back! A Dinosaur's Story, The Land Before Time and An American Tail)
- Bartholomew - Fievel (An American Tail)