- Ratigan - Cat R. Waul (An American Tail: Fievel Goes West) (the main antagonist)
- Basil - Tigger (Pooh) (the protagonist)
- Dr. Dawson - Winnie the Pooh (Pooh) (the deuteragonist)
- Olivia - Kessie (Pooh) (the tritagonist)
- Mrs. Judson - Kanga (Pooh) (a supporting character)
- Felicia - Dragon the Cat (The Secret of NIMH) (the secondary antagonist)
- Fidget - Stan Woozle (The New Adventures of Winnie the Pooh) (the former tertiary antagonist)
- Stan Woozle's extra - Heff Heffalump (The New Adventures of Winnie the Pooh) (the quartenary antagonist)
- Ratigan's gang - Cat R. Waul's henchmen (An American Tail: Fievel Goes West) (supporting antagonists)
- Hiram Flaversham - Rabbit (Pooh) (the tetartagonist)
- Toby - Eeyore (Pooh) (a supporting character)
- Bartholomew - Chula the Tarantula (An American Tail: Fievel Goes West) (a minor character)
- Barmaid - Mama Heffalump (Pooh) (a minor character)
- Juggling Octopus - Tiger (An American Tail) (a minor character)
- Frog and Salamander on Bike - Owl and Gopher (Pooh) (minor characters)
- Miss Kitty - Tanya (An American Tail) (a minor character)
- Miss Kitty's Sisters - Cholena (An American Tail: The Treasure of Manhattan Island) and Nellie Brie (An American Tail: The Mystery of the Night Monster) (minor characters)
- Queen Moustoria - Miss Kitty (An American Tail: Fievel Goes West) (a major character)
In London, circa 1897, a young Bird named Kessie is celebrating her birthday with her Rabbit father, Rabbit. Suddenly, Heff, a heffalump, barges in, and after a brief struggle, disappears with Rabbit. Heff takes Rabbit to his boss, Cat R. Waul who commands him to create a clockwork robot which mimics the Queen of the Mice so Waul can rule England. Rabbit initially refuses to participate in the scheme, whereupon Waul threatens to harm Kessie.
Kessie searches to find Tigger of Baker Street, a world-famous detective and Cat R. Waul's archnemesis. Returning to London after a tour of duty in Afghanistan, Dr. David Q. Winnie the Pooh Bear stumbles upon Kessie, and helps her find Tigger's residence. At first, Tigger is reluctant, but when Kessie mentions Heff and his woozle sidekick Stan, Tigger now realizes his chance to capture Waul. Tigger and Pooh take Eeyore, Sherlock Holmes's pet donkey, to track Stan and Heff's scent, where they find them in a toyshop stealing clockwork mechanisms and toy soldiers' uniforms. Stan and Heff later trap Kessie by ambushing her from inside a toy cradle. Tigger and Pooh pursue Stan and Heff, but become entangled and fall behind. While searching the shop, Pooh discovers Heff's checklist, to which Tigger does some chemical tests to discover the list came from a riverfront near the Thames. Tigger and Pooh disguise themselves as cowboys and go into a tavern called the "Rat Trap" and follow Heff to Waul's headquarters. They are caught, and Waul ties them to a spring-loaded mousetrap connected with a Rube Goldberg machine. Waul sets out for Buckingham Palace, where Heff and his accomplices kidnap the queen. Tigger, along with Pooh, deduces the trap's weakness and escape just in time.
Back at Buckingham Palace, Waul forces Rabbit to operate the toy Queen, while the real Queen is taken to be fed to Wooster and Hawk. The toy Queen declare Waul the ruler of all London, and he announces his tyrannical plans for his new "subjects". Just then, Tigger, Pooh and Kessie save Rabbit and the real Queen, and apprehend Heff and Stan (along with Waul's other henchmen). Tigger seizes control of the mechanical queen, making it denounce Waul as a fraud and tyrant while breaking into pieces. The crowd, enraged by Waul's treason, start climbing on him and defeating his shanty guards (meanwhile, Eeyore chases down Hawk and delivers her straight to the Royal Guard Dogs). Waul frees himself and escapes on his dirigible with Stan and Heff, holding Kessie hostage. Tigger, Pooh, and Rabbit create their own craft with a matchbox and some small helium-filled balloons, held under the Union Jack. Waul throws Heff (who can't fly) into the Thames River to his death while, Stan then attempts to drive the dirigible himself. Tigger jumps on to the dirigible to confront Waul, causing him to crash straight into the Big Ben. Inside the clock, Tigger manages to get Waul's cape stuck on some gears and rescues Kessie and Stan and safely delivers her to Rabbit. Waul, however breaks free and nearly kills Tigger (even knocking him to the dirigible) until the clock bell strikes 10:00 and Waul falls to his death, taking Tigger with him. However, Tigger manages to grab the wreckage of Cat R. Waul's dirigible and save himself from falling just in time.
Back at Baker Street, Stan, Tigger and Pooh recount their adventures, as well as the queen's gratitude for saving her life. Afterwards, the Flavershams leave to catch their train. As Pooh tries to leave, a distraught new client persuades Stan, Tigger and Pooh to help her. Finally, Tigger proclaims Pooh to be his "trusted associate, Doctor Pooh Bear, with whom I do all my cases" and redeems and promotes Stan to be his "reporter after he defeats all his nemesises".
- Tigger/Basil: Don't worry, old fellow. Uh, it's not entirely hopless. Pooh Bear, we'll get her back.
- Pooh/Dawson: And so we will. Do-Do you think there's a chance?
- Tigger/Basil: There's always a chance, Doctor, as long as one can think.
- This is the first movie-spoof for TheBeckster1000.
- This is also the first movie-spoof Winnie the Pooh appears in.
- The Great Tigger Detective is almost like a Winnie the Pooh movie.
- FBI Warning
- Coming Attractions Bumper
- The Breakfast Club trailer
- kairel in wonderland trailer
- Littlefoot's Adventures of Aladdin (English-Language Version) Trailer
- Benny, Leo, and Johnny's adventures of The Lion King Trailer
- Pooh's Adventures of The Future is Wild trailer
- Feature Presentation
- The Great Tigger Detective Intro - Don't Forget to Watch the Movie (My Version)
- The Great Tigger Detective part 1 - Kidnapped/"Main Titles"
- The Great Tigger Detective part 2 - Pooh (Dawson) Finds Kessie (Olivia)
- The Great Tigger Detective part 3 - Enter Tigger (Basil)
- The Great Tigger Detective part 4 - Enter Cat R. Waul (Ratigan)
- The Great Tigger Detective part 5 - "The World's Greatest Criminal Mind"
- The Great Tigger Detective part 6 - The Unusual Footprints/Here's Eeyore (Toby)
- The Great Tigger Detective part 7 - At the Toy Store
- The Great Tigger Detective part 8 - Heff and Stan (Fidget) Kidnap Kessie (Olivia)/The Chase
- The Great Tigger Detective part 9 - 'There's Always a Chance, Doctor'/The Reunion
- The Great Tigger Detective part 10 - Cat R. Waul's Plan
- The Great Tigger Detective part 11 - Tigger's Observation
- The Great Tigger Detective part 12 - At the Pub
- The Great Tigger Detective part 13 - "Let Me Be Good To You"
- The Great Tigger Detective part 14 - The Bar Fight/Following Stan
- The Great Tigger Detective part 15 - Tigger and Cat R. Waul's Confrontation
- The Great Tigger Detective part 16 - Cat R. Waul's Trap/"Goodbye So Soon"/The Queen's Doom
- The Great Tigger Detective part 17 - 'We Set The Trap Off Now!'
- The Great Tigger Detective part 18 - At Buckingham Palace
- The Great Tigger Detective part 19 - The Big Ben Chase (Part 1)
- The Great Tigger Detective part 20 - The Big Ben Chase (Part 2)
- The Great Tigger Detective part 21 - 'I'll Never Forget You'/A New Case
- The Great Tigger Detective part 22 - End Credits; "Goodbye So Soon (Reprise)"