- Florenz Ziegfeld, Jr. as Mario
- Billie Burke as Princess Daisy
- Anna Held as Princess Peach
- Billings as Luigi
- Fannie Brice as Princess Rosalina
- Audrey Dane as Birdo
- Sampson as Yoshi
- Ray Bolger as Toad
- Sidney as Wario
- Dr. Ziegfeld as Waluigi
- Sandow as Bowser Jr.
- Harriet Hector as Wendy O. Koopa
- Mary Lou as Sultan Pasbah
- Erlanger as Bowser
- Customer as Donkey Kong
- Pierre as Diddy Kong
- and more
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