- John Longridge as Mickey Mouse
- Professor James Hunter as Goofy
- Nancy Hunter as Daisy Duck
- Elizabeth Hunter as Minnie Mouse
- Peter Hunter as Donald Duck
- The Hermit as Pluto
- James MacKenzie as Professor Ludwig Von Drake
- Nell MacKenzie as Clarabelle Cow
- Mrs. Oakes as Ortensia
- Bert Oakes as Chip
- Bodger as Dale
- Luath as Horce Horsecollar
- Tao as Huey Duck
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