- Dr. Alec Considine as Peter Griffin
- Dr. John Paul Otis as Chris Griffin
- Lisa Cardigan as Lois Griffin
- Dr. Lew Worship as Brian Griffin
- Gloria as Meg Griffin
- Dr. Sid Lackland as Glenn Quagmire
- Dr. Sidney Wohl as Dr. Hartman
- Dr. Dominic Riccio as Jesus
- Dr. Madolyn Bruckner as Connie D'Amico
- Mildred as Dr. Amanda Rebecca
- Nurse Didi Loomis as Donna Tubbs-Brown
- Mrs. Emma Auer as Jillian
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