- Dr. Alec Considine as Shrek
- Dr. John Paul Otis as Donkey
- Lisa Cardigan as Princess Fiona
- Dr. Lew Worship as Puss in Boots
- Gloria as Queen Lillian
- Dr. Sid Lackland as Pinocchio
- Dr. Sidney Wohl as The Big Bad Wolf
- Dr. Dominic Riccio as Magic Mirror
- Dr. Madolyn Bruckner as Sleeping Beauty
- Mildred as Doris
- Nurse Didi Loomis as Snow White
- Mrs. Emma Auer as Dragon
- and more
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