- Elroy Jetson as Mario
- Astro the Dog as Luigi
- George Jetson as Wario
- Mr. Cogswell as Waluigi
- Orbitty as Yoshi
- Judy Jetson as Peach
- Jane Jetson as Daisy
- Rosie the Robot Maid as Rosalina
- Mrs. Spacely as Toadette
- Arthur Spacely as Toad
- Mugsy Megatron as Donkey Kong
- Knuckles Nuclear as Diddy Kong
- Mr. Spacely as Bowser
- The Gripfather as Bowser Jr.
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