- Elroy Jetson as Mario
- Orbitty as F.L.U.D.D
- Judy Jetson as Princess Peach
- Astro the Dog as Toadsworth
- George Jetson as Red Toad
- Henry Orbit as Yellow Toad
- Montague Jetson as Green Toad
- Mac as Blue Toad
- Sam Spacely as Purple Toad
- Arthur Spacely as Yoshi
- Mr. Spacely as Bowser
- Mr. Cogswell as Bowser Jr./Shadow Mario
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