- Emma Wilder as Jessie the Cowgirl
- Noah Wilder as Woody
- David Wilder as Buzz Lightyear
- Jo Wilder as Bo Peep
- Larry White as Mr. Potato Head
- Naomi Schwartz as Mrs. Potato Head
- FBI Special Agent Nathaniel Broadman as Emperor Zurg
- FBI Task Force Agent as Sid Phillips
- Sheila Broadman as Mrs. Davis
- Harry Jones as Andy Davis
- Wendy as Hannah Phillips
- Lena as Barbie
- Brian Greene as Rex (cameo)
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