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- Chip as Kermit the Frog
- Tillie as Miss Piggy
- Rokko (from The Pebble and the Penguin) as Fozzie Bear
- Eric as The Great Gonzo
- The Toy Monkey as Rizzo the Rat
- Doc as Rowlf the Dog
- Rollo the Clown as Dr. Teeth
- Pete as Sam the Eagle
- Thomas (from Thomas and Friends) as Dr. Bunsen Honeydew
- Percy (from Thomas and Friends) as Beaker