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- Kitty Katswell (from T.U.F.F. Puppy) as Ariel
- Dudley Puppy (from T.U.F.F. Puppy) as Prince Eric
- Timmy Turner (from The Fairly Oddparents) as Flounder
- Yogi Bear (from Yogi Bear) as Sebastian
- Mr. Small (from The Amazing World of Gumball) as King Triton
- Princess Bubblegum (Adventure Time), Penny (The Amazing World of Gumball), Yo (Fanboy and Chum), Beth (Total Drama), Susan (Johnny Test), and Jazz Fenton (Danny Phantom) as Ariel's Sisters
- Cruella De Vil (101 Dalmatians) as Ursula
- Quick Draw McGraw as Scuttle