- Colonel Thompson as Mario
- US Army Ranger as Luigi
- Private Arthur "Dutch" Schultz as Wario
- John Steele as Waluigi
- Private Harris as Toad
- Captain Frank as Bowser
- US Army Ranger Major as Bowser Jr.
- US Army Ranger as Donkey Kong
- Major General Robert Haines as Oogtar
- Madame Barrault as Princess Peach
- Maid as Princess Daisy
- Brigadier General Theodore Roosevelt Jr. as Detective Herlock Solmes
- and more
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