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- Penelope as Matilda
- Dougal as Narrator
- Ermintrude as Lavander
- The Alarm Clock (Female) as Hortensia
- Rosalie as Amanda Thripp
- Brian as Bruce Bogtrotter
- Florence as Miss Honey (Florence and Miss Honey are both Beautiful and Friendly)
- Madame Blue as Miss Trunchbull (Madame Blue and Miss Trunchbull are both Have Loud Voices)
- The Train (Female) as Cookie
- Buxton as Harry Wormwood
- The Music Box (Female) as Zinnia Wormwood
- Paul as Mike Wormwood