- Sidney Stratton as Mario
- Daphne Birnley as Princess Peach
- Alan Birnley as Luigi
- Michael Corland as Yoshi
- Sir John Kierlaw as Toadsworth
- Cranford as Toad
- Hoskins as Oogtar
- Bertha as Princess Daisy
- Frank as Wario
- Harry as Diddy Kong
- Wilkins as Mallow
- and more
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