- Professor Harold Hill as Mickey Mouse
- Marian Paroo as Minnie Mouse
- Marcellus Washbum as Donald Duck
- Mayor George Shinn as Pluto
- Eulalie Mackenchie Shinn as Daisy Duck
- Mrs. Paroo as Clarabelle Cow
- Tommy Dijilas as Chip
- Zaneeta Shinn as Clara Cluck
- Winthrop Paroo as Dale
- Charlie Cowell as Oswald the Lucky Rabbit
- Constable Locke as Goofy
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