- Sylvester as March Hare
- Margaret as Alice
- Featherhead as Mad Hatter
- La Roche as Tweedledum
- The Hook as Tweedledee
- Pedro as The Dormouse
- Proprietor (Bucket of Blood) as The Dodo
- Landlady as Alice's Sister
- Don Jose as The King of Hearts
- Mr. Pelly as Cheshire Cat
- Alonzo as Caterpillar
- Captain of "Mary Ann" as Doorknob
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