• Sylvester as March Hare
  • Margaret as Alice
  • Featherhead as Mad Hatter
  • La Roche as Tweedledum
  • The Hook as Tweedledee
  • Pedro as The Dormouse
  • Proprietor (Bucket of Blood) as The Dodo
  • Landlady as Alice's Sister
  • Don Jose as The King of Hearts
  • Mr. Pelly as Cheshire Cat
  • Alonzo as Caterpillar
  • Captain of "Mary Ann" as Doorknob

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