- The Queen as Gertrude McFuzz
- Prime Minister Tony Blair as The Cat in the Hat
- Prince Philip as King Derwin
- Cherie Blair as Daisy-Head Mayzie
- Charles, Prince of Wales as Mack the Turtle
- Robin Janvrin as Sam-I-Am
- Queen Elizabeth as Jane Kangaroo
- Stephen Lamport as Mr. Knox
- Alastair Campbell as Fox in Socks
- Lord Airlie as Mr. Brown
- Equerry as Norval the Fish
- Janbrin's Secretary as Cindy Lou Who
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