- The Queen as Princess Peach
- Prime Minister Tony Blair as Mario
- Prince Philip as Luigi
- Cherie Blair as Princess Rosalina
- Charles, Prince of Wales as Toad
- Robin Janvrin as Yoshi
- Queen Elizabeth as Princess Daisy
- Stephen Lamport as Wario
- Alastair Campbell as Donkey Kong
- Lord Airlie as Bowser
- Equerry as Iggy Koopa
- Janbrin's Secretary as Birdo
- and more
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