• Dr. Frank N. Furter as Mario
  • Janet Weiss as Pauline
  • Brad Majors as Luigi
  • Riff Raff as Toad
  • Magenta as Princess Daisy
  • Columbia as Princess Peach
  • Dr. Everett V. Scott as Bowser
  • Rocky Horror as Donkey Kong
  • Eddie as Yoshi
  • The Ciminologist as Wario
  • Ralph Hapschatt as Waluigi
  • Betty Munroe Hapschatt as Princess Rosalina

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