- Dr. Frank N. Furter as Spongebob
- Janet Weiss as Sandy Cheeks
- Brad Majors as Patrick Star
- Riff Raff as MermaidMan
- Magenta as Princess Mindy
- Columbia as Pearl Krabs
- Dr. Everett V. Scott as Plankton
- Rocky Horror as Squidward
- Eddie as Mr. Krabs
- The Ciminologist as BarnacleBoy
- Ralph Hapschatt as Squilliam Fancyson
- Betty Munroe Hapschatt as Mrs. Puff
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