- Laura Reynolds as Princess Peach
- Dr. Edward Hewitt as Mario
- Claire Hewitt as Princess Rosalina
- Cos Erickson as Yoshi
- Ward Hendricks as Luigi
- Larry Brant as Oogtar
- Judge Thompson as Toadsworth
- Walter Robinson as Toad
- Paul Sutcliff as Waluigi
- Danny Reynolds as Baby Mario
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