- Duke as Paddington Bear
- Gru (from Despicable Me 2) as Mr. Brown
- Lucy (from Despicable Me 2) as Mrs. Brown
- Antonio (from Despicable Me 2) as Jonathan Brown
- Margo (from Despicable Me 2) as Judy Brown
- Ted Wiggins (from Dr. Seuss' The Lorax) as David Russell
- Katie's Grandma as Mrs. Bird
- Fred as Mr. Gruber
- Herb Overkill (from Minions) as Mr. Curry
- Scarlet Overkill (from Minions) as Madame Helenka
- Chloe as Aunt Lucy
- Morty and McGurk (from Dr. Seuss' The Lorax) as Burglars
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