• Josh Brent as Mickey Mouse
  • Nicole 'Nikki' Brent as Minnie Mouse
  • Detective Superintendent Frank Hanbury as Ludwig Von Drake
  • Charles Standish as Horace Horsecollar
  • Detective Inspector Tom Henderson as Dewey Duck
  • Helen Standish as Clarabelle Cow
  • Dr. Alan Richford as Louie Duck
  • Clive Lang as Pete
  • Ralph Beldon as Pluto
  • Brinton as Mortimer Mouse

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