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- Lillie Lightship (from TUGS) as Mrs. Brisby
- Ten Cents (from TUGS) as Jonathan
- Toby as Mr. Ages
- Grampus (from TUGS) as Timmy
- Percy as Martin
- Mavis as Teresa
- A Green Coach as Cynthia
- Jeremy as Jeremy
- Harold as Miss Right
- Tiger Moth as The Great owl
- Edward as Jenner
- Evil Percy as Evil Martin