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- Numbuh 4 as Scooby Doo
- Numbuh 2 as Shaggy
- Numbuh 5 as Velma
- Stan Marsh as Fred
- Wendy Testaburger as Daphne (Wendy Testaburger and Daphne are both Formerly Voiced By Mary Kay Bergman)
- Numbuh 86 as Thorn (Numbuh 86 and Thorn are both Voiced By Jennifer Hale)
- Numbuh 3 as Dusk
- Lisa Simpson as Luna
- Gregory Of Yardale as Ben Ravencroft
- Lindsay Naegle as Sarah Ravencroft (Lindsay Naegle and Sarah Ravencroft are both Voiced By Tress MacNeille)