- Homer as Joker
- Bart as Penguin
- Marge as Catwoman
- Lisa as Poison Ivy
- Ned Flanders as Riddler
- Snake Jailbird as Bane
- Professor Frink as Professor Hugo Strange
- Groundskeeper Willie as Killer Croc
- Barney as Lunkhead
- Edna Krabappel as Madame X
- Apu as King Tut
- Krusty as The Mad Hatter
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