- Principal Skinner as Manfred the Mammoth
- Bart as Sid the Sloth
- Homer as Diego the Saber-Tooth Tiger
- Milhouse as Scrat the Squirrel
- Edna Krabappel as Ellie the Mammoth
- Lisa as Peaches the Mammoth
- Rod and Todd as Crash & Eddie the Opossums
- Carl as Buck
- Mr. Burns as Captain Gutt
- Marge as Shira
- Barney as Squint the Rabbit
- Ralph as Flint
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