- Dr. Christopher Maitland as Mario
- Anthony Marco as Luigi
- Sir Matthew Phillips as Oogtar
- Jane Maitland as Princess Peach
- Inspector Wilson as Wario
- Police Surgeon as Donkey Kong
- Bert Travers as Toad
- Auctioneer as Toadsworth
- Dr. Londe as Mallow
- French Girl as Princess Daisy
- Pierre as Yoshi
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