• Ben Powell as Mario
  • Kate Powell as Princess Peach
  • Steve Powell as Luigi
  • Uncle George as Toad
  • Felicity Twitchell as Princess Daisy
  • Miles Thorpe as Yoshi
  • Gloria Tritt as Birdo
  • Fess Dorple as Toadette
  • Ebenezer Twitchell as Detective Herlock Solmes
  • Rabbit Warren as Donkey Kong
  • Felicity's Father as Toadsworth
  • Booper Mellish as Mallow
  • Mother as Princess Rosalina
  • Jenny Pruitt as Wendy O. Koopa
  • Dr. Frieden as Oogtar

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