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- Young Tarzan (from Tarzan) as Young Simba
- Dee-Dee (from Dexter's Laboratory) as Young Nala
- Adult Tarzan (from Tarzan) as Adult Simba
- Jane (from Tarzan) as Adult Nala
- Kerchak (from Tarzan) as Mufasa
- Kala (from Tarzan) as Sarabina
- Clayton (from Tarzan) as Scar
- Sylvester and Tantor (from Looney Tunes Tarzan) as Timon and Pumbaa
- Shenzi, Banzai and Ed as himself
- Maxie (from Tarzan II) as Rafiki
- Sife (from Tarzan II) as Sarafina