- Michael "Mike" O'Halloran as Mario
- Doug Roberts as Yoshi
- James "Jim" Duncan as Wario
- Susan Franklin as Princess Peach
- Harlee Claiborne as Luigi
- Patty Duncan Simmons as Birdo
- Roger Simmons as Donkey Kong
- Lisolette Mueller as Princess Daisy
- Harry Jernigan as Toadsworth
- Lorrie as Toadette
- Mayor Robert "Bob" Ramsay as Bowser
- Kappy as Baby Mario
- and more
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