- Edward Malus as Mario
- Sister Smmersisle as Wendy O. Koopa
- Sister Willow Woodward as Princess Peach
- Sister Honey as Cackletta
- Dr. T.H. Moss as Toad
- Sister Rose as Toad
- Sister Beech as Princess Daisy
- Sister Oak as Toadette
- Sister Violet as Birdo
- Rowan Moodward as Princess Rosalina
- Officer Pete as Donkey Kong
- Ivy as Oogtar
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