- Peter Rabbit as Mario
- Mr. Jeremy Fisher as Luigi
- Benjamin Bunny as Yoshi
- Jemima Puddle-Duck as Princess Peach
- Mrs. Tiggy-Winkle as Princess Daisy
- Flopsy as Birdo
- Johnny Town Mouse as Oogtar
- Tom Kitten as Toad
- Kep as Toadsworth
- Mrs. McGregor as Wendy O. Koopa
- Mr. McGregor as Bowser
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