• Peter Rabbit as Mario
  • Mr. Jeremy Fisher as Luigi
  • Benjamin Bunny as Yoshi
  • Jemima Puddle-Duck as Princess Peach
  • Mrs. Tiggy-Winkle as Princess Daisy
  • Flopsy as Birdo
  • Johnny Town Mouse as Oogtar
  • Tom Kitten as Toad
  • Kep as Toadsworth
  • Mrs. McGregor as Wendy O. Koopa
  • Mr. McGregor as Bowser

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