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- Fireman Sam as Basil
- Kenny as Dr. Dawson
- Noddy as Olivia Flaversham
- Postman Pat as Hiram Flaversham
- Wendy Testaburger as Mrs. Judson
- Cartman as Fidget
- Mario as Toby
- Paddington Bear as Bill
- Meg Griffin as Felisha
- Lois Griffin as The Queen Mouse
- Stewie Griffin as Professor Ratigan