- Molly Donahue as Princess Rosalina
- Terence Donahue as Mario
- Tim Donahue as Luigi
- Katy Donahue as Princess Daisy
- Victoria Hoffman as Princess Peach
- Lew Harris as Toad
- Steve Donahue as Diddy Kong
- Charles Gibbs as Yoshi
- Eddie Dugan as Wario
- Father Dineen as Donkey Kong
- Marge as Birdo
- Hatcheck Girl as Toadette
- Lilliam Sawyer as Waluigi
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