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- Thomas (from Thomas & Friends) as Mr. Conductor (Ringo Starr)
- Percy (from Thomas & Friends) as Mr. Conductor (George Carlin)
- Edward (from Thomas & Friends) as Mr. Conductor (Michael Angelis)
- Ten Cents (from TUGS) as Barney
- Eric Cartman (from South Park) as Ren
- Stan Marsh (from South Park) as Stimpy
- The Barney Characters as The Thomas Characters
- The Mario Characters as The Jay Jay Characters