- Sunshine (from TUGS) as Scooby Doo
- Thomas (from Thomas and Friends) as Shaggy
- Ten Cents (from TUGS) as Fred
- Lillie Lightship (from TUGS) as Daphne
- Emily (from Thomas and Friends) as Velma
- Boomer (from TUGS) as Scrappy Doo
- Foduck (from Theodore Tugboat) as Scooby Dum
- Diesel (from Thomas and Friends) as Villian
- Gordon or Boco (from Thomas and Friends) as A Police Officer
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