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- Gru - Thomas/Percy/Gordon/James/Henry/Edward/Duck/Himself
- Vector - Diesel/Diesel 10/Thomas/Edward/Duck/Himself
- Minions - Troublesome Trucks/Themselves
- Margo - Daisy/Mavis/Rosie/Emily/Himself
- Edith - Emily/Lady/Rosie/Himself
- Agnes - Mavis/Rosie/Caroline/Himself
- Dr.Neferio - Henry/Toby/Thomas/Himself
- Gru's Mom - Mavis/Daisy/Lady/Himself
- Ms.Hattie - Mavis/Emily/Lady Hatt/Molly/Himself
- Mr. Perkins - Diesel 10/Diesel/Bulgy/Gordon/Henry/James/Himself