• Emily as Doc Mcstuffins
  • Flora as Lambie
  • Thomas as Stuffy
  • Mavis as Hallie
  • Rusty as Squeakers
  • Henry as Chilly
  • Rosie as Susie Sushine
  • James as Hermie
  • Timothy as Ben
  • Marion as Anna
  • Bertie as Carl
  • Gordon as Fabio
  • Molly as Dolly
  • Toby as Morten
  • Stanley as Declan
  • Daisy as Emmie
  • Sally Seaplane (from TUGS) as Alma
  • Percy as Henry
  • Lillie Lightship (from TUGS) as Dr. Mcstuffins
  • Lady as Baby Suki
  • Caitlin as Dr. Reese
  • Sunshine (from TUGS) as Donny
  • Pugwash (from Theodore Tugboat) as Tamorina
  • Millie as Sabrina
  • Bluenose (from Theodore Tugboat) as Bubble Monkey
  • Ten Cents (from TUGS) as Mr. Mcstuffins
  • Madge as Florence
  • Emily (from Theodore Tugboat) as Suki's Mother
  • Isobella as Tisha
  • Rheneas as Tobias
  • Hiro as Santa Claus
  • Skarloey as Little Jack
  • Pearl (from TUGS) as Tamara
  • Georgia (from The Little Engine That Could/Casey Jr. and Friends) as Rescue Ronda
  • Hank as The Dude
  • Old Slow Coach as Maddie
  • Whiff as Ian

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