Cast[]
- Emily as Doc Mcstuffins
- Flora as Lambie
- Thomas as Stuffy
- Mavis as Hallie
- Rusty as Squeakers
- Henry as Chilly
- Rosie as Susie Sushine
- James as Hermie
- Timothy as Ben
- Marion as Anna
- Bertie as Carl
- Gordon as Fabio
- Molly as Dolly
- Toby as Morten
- Stanley as Declan
- Daisy as Emmie
- Sally Seaplane (from TUGS) as Alma
- Percy as Henry
- Lillie Lightship (from TUGS) as Dr. Mcstuffins
- Lady as Baby Suki
- Caitlin as Dr. Reese
- Sunshine (from TUGS) as Donny
- Pugwash (from Theodore Tugboat) as Tamorina
- Millie as Sabrina
- Bluenose (from Theodore Tugboat) as Bubble Monkey
- Ten Cents (from TUGS) as Mr. Mcstuffins
- Madge as Florence
- Emily (from Theodore Tugboat) as Suki's Mother
- Isobella as Tisha
- Rheneas as Tobias
- Hiro as Santa Claus
- Skarloey as Little Jack
- Pearl (from TUGS) as Tamara
- Georgia (from The Little Engine That Could/Casey Jr. and Friends) as Rescue Ronda
- Hank as The Dude
- Old Slow Coach as Maddie
- Whiff as Ian