• Thomas as Duke Nukem
  • Emily/Rosie/Lady/Mavis/Molly/Daisy as the Babes
  • Sir Topham Hatt as General Graves
  • Percy as Assault Trooper
  • James as Assault Captain
  • Arry/Bert/Duck/Oliver as Assault Enforcer
  • Gordon/Edward/Henry/Sir Handel as Pig Cop
  • Belle as Pig in a Dress (Pig Cop Girl)
  • Flynn as Tricera Cop
  • BoCo as Assault Commander
  • The Troublesome Trucks as Octabrain and the Protector Drones
  • Diesel as Dr. Proton
  • George as Battlelord
  • Diesel 10 as Cycloid Emperor
  • Bulgy as Overlord
  • Judge Judy the Diesel as Alien Queen