Cast[]
- Twilight Sparkle - Thomas/Duck/Mavis/Emily/James/Gordon/Henry/Edward/Molly/Neville/BoCo/Murdoch
- Pinkie Pie - Percy/James/Edward/Thomas/Henry/Rosie/Gordon
- Rarity - Henry/Gordon/James/Edward/Rosie/Emily/Skarloey/Thomas/Diesel
- Applejack - Toby/Gordon/James/Marion
- Fluttershy - Edward/Thomas/Duck/Molly/James/Henry/Gordon/BoCo/Neville
- Rainbow Dash - Duck/BoCo/Thomas/Gordon/James/Henry/Edward/Belle
- Apple Bloom - Oliver/Edward/James/Thomas/Gordon
- Scootaloo - Stepney/Duck/Thomas/Henry/Gordon/Henrietta
- Sweetie Belle - Toad/Edward/James/Gordon/Clarabel
- Spike - Phillip/Thomas/Edward
- Princess Celestia - Sir Topham Hatt/Lady Hatt
- Princess Luna - Mr. Percival/The Stationmaster's Wife
- Big Macintosh - Gordon/Murdoch
- Granny Smith - Flora/Edward
- Mayor Mare - Dowager Hatt/Mavis/Toby/Oliver/Duck
- Snips - Bill/James/Donald/Rheneas/Skarloey
- Snails - Ben/Edward/Douglas/Skarloey/Rheneas
- Mr. Cake - Connor/Edward/Duck
- Mrs. Cake - Caitlin/James/Thomas/Mavis/Emily/Edward
- Zecora - Caroline/Duncan/Oliver/Culdee (From RWS)
- Owlowiscious - Harold
- Angel - Bertie
- Gummy - Trevor
- Tank - Toad
- Winona - Cora (from RWS)
- Opalescence - Beatrice (from RWS)
- Discord - Diesel/Diesel 10/James/Gordon
- Shining Armor - Edward/James/Duck/Thomas
- Princess Cadence - Lady/Molly
- Filthy Rich - Henry/Gordon/Oliver/Murdoch/Spencer/Duck
- Diamond Tiara - Arry/Gordon/Bill
- Silver Spoon - Bert/Henry/Ben
- Dr. Hooves - Oliver/Duck/James/Thomas
- Derpy - Millie/Henry/James/Gordon/Terence/Stanley
- Cranky Doodle Donkey - James/Cranky
- Matilda - Old Slow Coach/Molly
- Spitfire - Miss Jenny/Oliver/Duncan
- Soarin - Duck/Stepney
- Braeburn - Toby/Duncan/Rusty/Bill/Ben/Arthur
- Chief Thunderhooves - Murdoch/Arthur/Sir Handel
- Prince Blueblood - Spencer/Duncan
- Cheese Sandwich - Salty/Spencer/James/Gordon/Conner
- Coco Pommel - Dennis
- Flash Sentry - Stephen/Conner/James/Gordon/Spencer
- Randolph - Duke
- Gilda - Daisy/Elizabeth
- Trixie - Elizabeth/Daisy/Diesel
- Flim - Splatter/Bill/Wilbert (From the RWS)
- Flam - Dodge/Ben/Sixteen (From the RWS)
- Garble - Bulgy/George
- King Sombra - Diesel 10/Diesel/James/D261/George
- Lord Tirek - D261/Diesel 10/Diesel
- Wind Rider - Sailor John/Jinty (From the RWS)
- Fancy Pants - BoCo
- Trouble Shoes - Derek/Gordon/Bill/Ben
- Princess Ember - Ashima/Frieda/Gina/Jinty (From the RWS)/Pug (From the RWS)/Edward
- Babs Seed - Duncan/Gordon/Edward/Skarloey/Rusty/Duck
- Thorax - Hector
- Maud Pie - Mavis/Duke
- Silver Shill - Paxton
- Trenderhoof - Neville
- Night Light - Donald
- Twilight Velvet - Pip (from RWS)
- Hondo Flanks - Douglas
- Cookie Crumbles - Emma (from RWS)
- Quibble Pants - Samson
- Iron Will - Bradford