• Thomas as Osmosis Jones
  • Gordon as Drix
  • Emily as Leah
  • James as Mayor
  • Eric Cartman (from South Park) as Tom Colonic
  • The Other Engines as Other Germs
  • Henry as Chief of Police
  • Troublsome Trucks as The Polices
  • The Canadian (from South Park) as The Dry Germs
  • Diesel 10, D261, Arry/Bert, Splatter, Dodge and Hector as Bad Germs
  • Blue Trucks as Flopper Germs
  • Percy and Rosie as News Report
  • Diesel as Thrax
  • Whiff as Frank
  • Duchess (from TUGS) as Mrs. Boyd
  • Mavis as Shane
  • Derek as Bob

See Also

Ad blocker interference detected!

Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers

Wikia is not accessible if you’ve made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected.