• Thomas as Samurai Jack
  • Diesel 10 as Aku
  • Donald/Douglas as The Scotsman
  • Devious Diesel as Demongo
  • Gordon as The Emperor
  • Emily as The Empress
  • Rosie as Ashi
  • D261 as Scaramouche
  • Daisy as The High Priestess
  • Bulgy as X-49
  • Murdoch as The Guardian
  • Mavis as The Scotsman's Wife
  • Henry as The Creature
  • Edward as Master Chu
  • Toby as Sir Rothchild
  • James as Spartok
  • Spencer as Da Samurai
  • Evil Thomas (A Fanmade Thomas Character) as Mad Jack/Inner Jack
  • Molly as Flora
  • Splatter, Dodge, and S.C.Ruffey as Aku's Evil Scientist
  • George as Aku's Therapist
  • Hector as Dominator
  • Troublesome Trucks as Minions of Aku
  • Arry and Bert as I and Am
  • Elizabeth as Princess Mira
  • Dennis as Tango Beast
  • Neville as Farting Dragon
  • Lady as The Fairy
  • Hank as African Chief
  • BoCo as The Lava Monster

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