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- Thomas as Scooby-Doo!
- Percy as Shaggy Rogers
- James a Fred Jones
- Emily as Daphne Blake
- Mavis as Velma Dinkley
- Spencer as Scrappy-Doo! (Billy can't be Scrappy: Billy is a hero and Scrappy is a Villain)
- Rabbit as Rabbit
- Diesel 10 as Scrappy Rex