• Percy as Scooby Doo
  • Thomas as Shaggy Rogers
  • Henry as Fred Jones
  • Emily as Daphne Blake
  • Rosie as Velma Dinkley
  • Gordon as Officer Wembley
  • Edward as Eric Staufer
  • Toby as Professor Kaufman
  • Diesel as Bill McLemore (both are the main villains)
  • Diesel 10 as The Phantom Virus
  • Arry and Bert as Moonmen
  • Horrid Lorries as Gladiator Skeletons
  • Hector as The Lion
  • Donald and Douglas as Woolly Mammoths
  • The Spiteful Brakevan as T-Rex
  • Bulstrode as Shark
  • S.C. Ruffey as Samuri
  • The Chinese Dragon as Dragon
  • Oliver as Cyber Scooby Doo
  • Duck as Cyber Shaggy Rogers
  • James as Cyber Fred Jones
  • Molly as Cyber Daphne Blake
  • Mavis as Cyber Velma Dinkley
  • George as The Creeper
  • Dodge as Jaguaro
  • D199 as Tar Monster
  • Old-Stuck Up as Gator Ghoul
  • Splatter as Old Iron Face

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