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- Thomas as Tubby the Tuba
- Percy as Pee-Wee the Piccolo
- James as The Herald
- Duck as the Frog
- Gordon as The Great Pepperino
- Emily as Celeste
- Mavis as The Haughty Violin
- Rosie as Mrs. Elephant
- Daisy as Mrs. Squeek
- Captain Star (from TUGS) as Narrator