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- Babs Bunny as Penelope
- Dr. Gene Splicer as The Hooded Claw
- Silas' Minions as The Bully Brothers
- Byron Basset as Snoozey
- Dizzy Devil as Zippy
- Gogo as Yak Yak
- Furball as Dum Dum
- Banjo Possum as Softy
- Little Beeper as Pockets
- Dr. Otto ScratchNSniff (from Animaniacs) as Clyde
- Shirley the Loon as Chugaboom